Check where it's connected.
MIO is dedicated Pins for PS. I don't know by heart which pins are avaialbe on any connector.
So in case it's PL, you must configure PS with EMIO end connect EMIO to PL IOs correctly.
In case it's MIO, you must configure PS to MIO GPIO (disable I2C if you want and you know that these MIO are available on connector).
But in any case you must regenerate XSA and all you software file --> fsbl, u-boot, linux .....
br
John